Endophytic microorganisms from Bauhinia monandra leaves: Isolation, antimicrobial activities and interaction with galactose-specific lectin BmoLL

Isolation of Endophytic Actinomycetes from Catharanthes roseus (L.) G. Don Leaves and Their Antimicrobial Activity

Endophytic actinomycetes were isolated from surface sterilized leaves of Catharanthes roseus (L.) G. Don offamily Apocynaceae. A total of 38 endophytic actinomycetes were recovered on Starch Casein Agar.Among the 38 isolates 20 morphologically different isolates were screened for antibacterial activity againstBacillus subtilis, Staphylococcus aureus, Pseudomonas aeruginosa, Pr...

A New Galactose-Specific Lectin from Clerodendrum infortunatum

Background: The ethno-medical significance of Clerodendrum genus raises the interest towards the characterization of its seed lectin by inexpensive and most effective technique.Objective: The focus of this study is the purification, characterization, and evaluation of the antioxidant and antiproliferative potential of a galactose-specific lectin from Cler...

Antimicrobial activities of endophytic fungi isolation from Ophiopogon japonicus

Carbohydrate binding activities of Bradyrhizobium japonicum. II. Isolation and characterization of a galactose-specific lectin

Extracts of Bradyrhizobium japonicum were fractionated on Sepharose columns covalently derivatized with lactose. Elution of the material that was specifically bound to the affinity column with lactose yielded a protein of Mr approximately 38,000. Isoelectric focusing of this sample yielded two spots with pI values of 6.4 and 6.8. This protein specifically bound to galactose-containing glycoconj...

Isolation of the Galactose-binding Lectin

Entamoeba histolytica adheres to human colonic mucus, colonic epithelial cells, and other target cells via a galactose (Gal) or N-acetyl-D-galactosamine (GalNAc) inhibitable surface lectin. Blockade of this adherence lectin with Gal or GalNAc in vitro prevents amebic killing of target cells. We have identified and purified the adherence lectin by two methods: affinity columns derivatized with g...